The effects of poly(dimethylsiloxane) surface silanization on the mesenchymal stem cell fate. (15th October 2014)

• Record Type: Journal Article
• Authors: Chuah, Yon Jin; Kuddannaya, Shreyas; Lee, Min Hui Adeline; Zhang, Yilei; Kang, Yuejun
• Is Part Of: Biomaterials science. Volume 3:Number 2(2015:Feb.)
• Page Start: 383
• Page End: 390
• Publication Date: 2014-10-15
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c4bm00268g
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Surface silanization with 3-aminopropyl triethoxy silane (APTES) ± glutaraldehyde (GA) enhanced the biocompatibility of poly(dimethylsiloxane) surfaces for long term cell culture investigation. Abstract : In recent years, poly(dimethylsiloxane) (PDMS)-based microfluidic devices have become very popular for on-chip cell investigation. Maintenance of mammalian cell adhesion on the substrate surface is crucial in determining the cell viability, proliferation and differentiation. However, the inherent hydrophobicity of PDMS is unfavourable for cell culture, causing cells to eventually dislodge from the surface. Although physically adsorbed matrix proteins can promote initial cell adhesion, this effect is usually short-lived. To address this critical issue, in this study, we employed (3-aminopropyl) triethoxy silane (APTES) and cross-linker glutaraldehyde (GA) chemistry to immobilize collagen type 1 (Col1) on PDMS. These modified surfaces are highly efficient to support the adhesion of mesenchymal stem cells (MSCs) with no deterioration of their potency. Significant changes of the native PDMS surface properties were observed with the proposed surface functionalization, and MSC adhesion was improved on PDMS surfaces modified with APTES + GA + Protein. Therefore, this covalent surface modification could generate a more biocompatible platform for stabilized cell adhesion. Furthermore, this modification method facilitated long-term cell attachment, which is favourable for successful induction of osteogenesis and cell sheet formation with an increased expression of osteogenic biomarkers and comparable extracellular matrix (ECM) constituent biomarkers, respectively. The surface silanization can be applied to PDMS-based microfluidic systems for long-term study of cellular development. Similar strategies could also be applied to several other substrate materials by appropriate combinations of self-assembled monolayers (SAMs) and ECM proteins.