Multifunctional PEI-entrapped gold nanoparticles enable efficient delivery of therapeutic siRNA into glioblastoma cells. (6th December 2016)

• Record Type: Journal Article
• Authors: Kong, Lingdan; Qiu, Jieru; Sun, Wenjie; Yang, Jia; Shen, Mingwu; Wang, Lu; Shi, Xiangyang
• Is Part Of: Biomaterials science. Volume 5:Number 2(2017:Feb.)
• Page Start: 258
• Page End: 266
• Publication Date: 2016-12-06
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c6bm00708b
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Multifunctional PEI-entrapped gold nanoparticles modified with RGD peptide via a PEG spacer enable efficient therapeutic siRNA delivery to glioblastoma cells. Abstract : RNA interference (RNAi) has been considered as a promising strategy for effective treatment of cancer. However, the easy degradation of small interfering RNA (siRNA) limits its extensive applications in gene therapy. For safe and effective delivery of siRNA, a novel vector system possessing excellent biocompatibility, highly efficient transfection efficiency and specific targeting properties has to be considered. In this study, we report the use of polyethyleneimine (PEI)-entrapped gold nanoparticles (Au PENPs) modified with an arginine-glycine-aspartic (Arg-Gly-Asp, RGD) peptide via a poly(ethylene glycol) (PEG) spacer as a vector for Bcl-2 (B-cell lymphoma-2) siRNA delivery to glioblastoma cells. The synthesized Au PENPs were well characterized. The efficiency of siRNA delivery was appraised by flow cytometry, confocal microscopy imaging, and the protein expression level. Our results revealed that the Au PENPs were capable of delivering Bcl-2 siRNA to glioblastoma cells with an excellent transfection efficiency, leading to specific gene silencing in the target cells (22% and 25.5% Bcl-2 protein expression in vitro and in vivo, respectively) thanks to the RGD peptide-mediated targeting pathway. The designed RGD-targeted Au PENPs may hold great promise to be used as a novel vector for specific cancer gene therapy applications.