Studies on antioxidative and immunostimulating fucogalactan of the edible mushroom Macrolepiota dolichaula. (2nd September 2015)

• Record Type: Journal Article
• Authors: Samanta, Surajit; Nandi, Ashis K.; Sen, Ipsita K.; Maity, Prasenjit; Pattanayak, Manabendra; Devi, K. Sanjana P.; Khatua, Somanjana; Maiti, Tapas K.; Acharya, Krishnendu; Islam, Syed S.
• Is Part Of: Carbohydrate research. Volume 413(2015)
• Page Start: 22
• Page End: 29
• Publication Date: 2015-09-02
• Subjects: Macrolepiota dolichaula -- Fucogalactan -- NMR spectroscopy -- Antioxidant -- Immunostimulation; Carbohydrates -- Periodicals; Chemistry, Organic -- Periodicals; Biochemistry -- Periodicals; Carbohydrates -- Periodicals; Chimie organique -- Périodiques; Glucides -- Périodiques; Biochemistry; Carbohydrates; Chemistry, Organic; Periodicals; Electronic journals; 507.78
• DOI: 10.1016/j.carres.2015.05.006
• Languages: English
• ISSNs: 0008-6215

Abstract

Abstract: A water soluble fucogalactan (PS-II) of an average molecular weight ∼1.2×10 5 Da was isolated from the aqueous extract of an edible mushroom Macrolepiota dolichaula . It was composed of fucose, galactose and 3-O-methyl galactose in a molar ratio of nearly 1:4:1. Structural characterization of PS-II was carried out using total hydrolysis, methylation analysis, Smith degradation, and 1D/2D NMR experiments. These results indicated that the proposed repeating unit of the PS-II had a backbone chain consisting of four (1→6)- linked α-d -Gal p residues, one residue methylated at O-3, and another one substituted at O-2 by (1→2)-α-d -Gal p residue, which is terminated with a α-l -Fuc p moiety. The PS-II exhibited the antioxidant properties in different in vitro test systems, and also showed in vitro macrophage activation in RAW 264.7 cell line as well as splenocyte and thymocyte activation in mouse cell culture medium. Graphical abstract: Highlights: A water soluble fucogalactan was isolated from the mushroom Macrolepiota dolichaula. Its structure was established by chemical and 1D/2D NMR experiments. This fucogalactan showed antioxidant properties in different in vitro test systems. This fucogalactan also exhibited immunoenhancing activity.