Complex formation of albumin with tricarbocyanine dyes containing phosphonate groups. Issue 11 (3rd October 2016)

• Record Type: Journal Article
• Authors: Kuzmin, Vladimir A.; Nekipelova, Tatiana D.; Podrugina, Tatyana A.; Golovina, Galina V.; Kostyukov, Alexey A.; Temnov, Viktor V.; Doroshenko, Irina A.; Radchenko, Eugene V.; Palyulin, Vladimir A.; Zefirov, Nikolay S.
• Is Part Of: Photochemical & photobiological sciences. Volume 15:Issue 11(2016:Nov.)
• Page Start: 1377
• Page End: 1384
• Publication Date: 2016-10-03
• Subjects: Photochemistry -- Periodicals; Photobiology -- Periodicals; 541.35
• DOI: 10.1039/c6pp00246c
• Languages: English
• ISSNs: 1474-905X

Abstract

Abstract : Novel indotricarbocyanine dyes bearing remote phosphonate groups show good binding with albumins. Abstract : The spectral characteristics, binding constants with bovine (BSA) and human serum albumin (HSA) and lifetimes of fluorescence in PBS and EtOH solutions and in the presence of BSA in PBS were measured for novel indotricarbocyanine dyes bearing remote phosphonate groups. These parameters are close to those for indocyanine green (ICG) indicating that the Coulomb interaction does not play a significant role in complex formation, and the binding is determined by the interaction of the dye polymethine chain with albumin. The fluorescence lifetimes of the complexes with BSA strongly indicate the formation of complexes of two types with different lifetimes. The complex with a longer fluorescence lifetime (740–800 ps) and major contribution (up to 88%) is bound to the more hydrophobic site and that with a shorter fluorescence lifetime (300–340 ps) to the more hydrophilic site.