Determination of eurycomanone in rat plasma using hydrophilic interaction liquid chromatography–tandem mass spectrometry for pharmacokinetic study. (26th September 2016)

• Record Type: Journal Article
• Authors: Rehman, Shaheed Ur; Choi, Min Sun; Han, Young Min; Kim, In Sook; Kim, Seung Hyun; Piao, Xiang‐Lan; Yoo, Hye Hyun
• Is Part Of: Biomedical chromatography. Volume 31:Number 4(2017:Apr.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-09-26
• Subjects: Eurycoma longifolia -- eurycomanone -- HILIC‐MS/MS -- pharmacokinetics -- plasma; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3831
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: In this study, a rapid, sensitive, and reliable hydrophilic interaction liquid chromatography–tandem mass spectrometry (HILIC‐MS/MS) method for the determination of eurycomanone in rat plasma was developed and validated. Plasma samples were pretreated with a protein precipitation method and quercitrin was used as an internal standard (IS). A HILIC silica column (2.1 × 100 mm, 3 μm) was used for hydrophilic‐based chromatographic separation, using the mobile phase of 0.1% formic acid with acetonitrile in gradient elution at a flow rate of 0.25 mL/min. Precursor–product ion pairs for multiple‐reaction monitoring were m / z 409.1 → 391.0 for eurycomanone and m / z 449.1 → 303.0 for IS. The linear range was 2–120 ng/mL. The intra‐ and inter‐day accuracies were between 95.5 and 103.4% with a precision of <4.2%. The developed method was successfully applied to the pharmacokinetic analysis of eurycomanone in rat plasma after oral dosing with pure compound and E. longifolia extract. The C max and AUC0– t, respectively, were 40.43 ± 16.08 ng/mL and 161.09 ± 37.63 ng h/mL for 10 mg/kg eurycomanone, and 9.90 ± 3.97 ng/mL and 37.15 ± 6.80 ng h/mL for E. longifolia extract (2 mg/kg as eurycomanone). The pharmacokinetic results were comparable with each other, based on the dose as eurycomanone.