Biobutanol production by Clostridium acetobutylicum using xylose recovered from birch Kraft black liquor. (January 2015)

• Record Type: Journal Article
• Authors: Kudahettige-Nilsson, Rasika L.; Helmerius, Jonas; Nilsson, Robert T.; Sjöblom, Magnus; Hodge, David B.; Rova, Ulrika
• Is Part Of: Bioresource technology. Volume 176(2015)
• Page Start: 71
• Page End: 79
• Publication Date: 2015-01
• Subjects: ABE fermentation -- Birch wood Kraft black liquor -- Lignin precipitation -- Fermentation inhibitors -- Detoxification; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.11.012
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Hardwood Kraft black liquor was successfully fractionated and hydrolyzed into xylose. Active carbon was effective to remove inhibitors in the hydrolyzate. Xylose recovery was 99–100% during active carbon detoxification. ABE production reached 1.8–2.1 g/L in xylose recovered from Kraft black liquor. Abstract: Acetone–butanol–ethanol (ABE) fermentation was studied using acid-hydrolyzed xylan recovered from hardwood Kraft black liquor by CO2 acidification as the only carbon source. Detoxification of hydrolyzate using activated carbon was conducted to evaluate the impact of inhibitor removal and fermentation. Xylose hydrolysis yields as high as 18.4% were demonstrated at the highest severity hydrolysis condition. Detoxification using active carbon was effective for removal of both phenolics (76–81%) and HMF (38–52%). Batch fermentation of the hydrolyzate and semi-defined P2 media resulted in a total solvent yield of 0.12–0.13 g/g and 0.34 g/g, corresponding to a butanol concentration of 1.8–2.1 g/L and 7.3 g/L respectively. This work is the first study of a process for the production of a biologically-derived biofuel from hemicelluloses solubilized during Kraft pulping and demonstrates the feasibility of utilizing xylan recovered directly from industrial Kraft pulping liquors as a feedstock for biological production of biofuels such as butanol.