Rheological properties, biocompatibility and in vivo performance of new hydrogel-based bone fillers. (3rd October 2016)

• Record Type: Journal Article
• Authors: Giannoni, Paolo; Villa, Federico; Cordazzo, Cinzia; Zardi, Luciano; Fattori, Paolo; Quarto, Rodolfo; Fiorini, Mauro
• Is Part Of: Biomaterials science. Volume 4:Number 11(2016:Nov.)
• Page Start: 1691
• Page End: 1703
• Publication Date: 2016-10-03
• Subjects: Biomedical materials -- Periodicals; 610.28
• DOI: 10.1039/c6bm00478d
• Languages: English
• ISSNs: 2047-4830

Abstract

Abstract : Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder, cancellous chips and demineralized bone matrix granules, were compared in vitro and in vivo . Abstract : Three different heterologous substitutes for bone regeneration, manufactured with equine-derived cortical powder (CP), cancellous chips (CC) and demineralized bone matrix granules (DBM), were compared in in vitro and in vivo settings. We tested: a commercially available bone paste (Osteoplant-Activagen™, consisting of aqueous collagenous carrier, CP, DBM; named A); a second-generation injectable paste (20 kDa polyethylene glycol/hydroxypropyl-methyl cellulose-based hydrogel, CP, DBM; B); a pre-formed bone filler (400 kDa polyethylene oxide/hydroxypropyl-methyl cellulose-based hydrogel, CP, CC, DBM; C). Vitamin C acted as a visco-modulator during C and B β-rays sterilization, modifying graft injectability. For each filler, we examined dissolution in culture medium, gene expression of the substitute-exposed osteogenically-induced human bone marrow stromal cells (hBMSC), and performance in a rabbit bone defect model. A dissolved after 1 h, while fragmentation of B peaked after 8 h. C remained unaltered for 2 days, but affected the microenvironmental pH, slowing the proliferation of exposed cells. B-exposed hBMSC overexpressed bone sialoprotein, osteocalcin and RUNX2. For all fillers histological results evidenced bridged lesion margins, marrow replenishment and bone-remodeling. However, B-treated lesions displayed a metachromatic type II collagen-rich matrix with prehypertrophic-like cells, matching the in vitro expression of cartilage-specific markers, and suggesting a possible application of B/C double-layer monolithic osteochondral plugs for full-thickness articular defects.