Bacterium‐Expressed dsRNA Downregulates Microsporidia Nosema bombycis Gene Expression. (27th July 2016)

• Record Type: Journal Article
• Authors: Pan, Qiuling; Wang, Ling; Dang, Xiaoqun; Ma, Zhengang; Zhang, Xiaoyan; Chen, Shiliang; Zhou, Zeyang; Xu, Jinshan
• Is Part Of: Journal of eukaryotic microbiology. Volume 64:Number 2(2017)
• Page Start: 278
• Page End: 281
• Publication Date: 2016-07-27
• Subjects: ADP/ATP carrier protein -- domestic silkworm -- RNA interference; Protista -- Periodicals; Eukaryotic cells -- Periodicals; Microbiology -- Periodicals; 579.05
• DOI: 10.1111/jeu.12346
• Languages: English
• ISSNs: 1066-5234

Abstract

Abstract: The microsporidia Nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. Here, we describe the use of Escherichia coli HT115 strain (DE3) to express double‐strand RNAs targeting the gene encoding ADP/ATP protein in N. bombycis . The results showed that dsRNAs deferentially suppressed the gene expression during N. bombycis infection in the silkworm, and the effect waned gradually. Our results, for the first time, provide a tool to utilize the dsRNA expressed by recombinant E. coli to control the pebrine disease of the domestic silkworm.