Transcriptome and proteome analyses reveal complex mechanisms of reproductive diapause in the two‐spotted spider mite, Tetranychus urticae. Issue 2 (21st December 2016)

• Record Type: Journal Article
• Authors: Zhao, J.‐Y.; Zhao, X.‐T.; Sun, J.‐T.; Zou, L.‐F.; Yang, S.‐X.; Han, X.; Zhu, W.‐C.; Yin, Q.; Hong, X.‐Y.
• Is Part Of: Insect molecular biology. Volume 26:Issue 2(2017:Apr.)
• Page Start: 215
• Page End: 232
• Publication Date: 2016-12-21
• Subjects: diapause -- Ca2+ signalling -- Tetranychus urticae -- proteome -- transcriptome; Insects -- Molecular aspects -- Periodicals; 595.7
• DOI: 10.1111/imb.12286
• Languages: English
• ISSNs: 0962-1075

Abstract

Abstract: Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown. Here, to better understand this process, we examined diapause‐associated genes in the two‐spotted spider mite, Tetranychus urticae, by comparing the transcriptomes and proteomes of early diapausing and reproductive adult females. Amongst genes underlying diapause revealed by the transcriptomic and proteomic data sets, we described the noticeable change in Ca 2+ ‐associated genes, including 65 Ca 2+ ‐binding protein genes and 23 Ca 2+ transporter genes, indicating that Ca 2+ signalling has a substantial role in diapause regulation. Other interesting changes in diapause included up‐regulation of (1) glutamate receptors that may be involved in synaptic plasticity changes, (2) genes involved in cytoskeletal reorganization including genes encoding each of the components of thick and thin filaments, tubulin and members of integrin signalling and (3) genes involved in anaerobic energy metabolism, which reflects a shift to anaerobic energy metabolism in early diapausing mites.