Downregulation of B‐myb promotes senescence via the ROS‐mediated p53/p21 pathway, in vascular endothelial cells. (23rd November 2016)

• Record Type: Journal Article
• Authors: Zhou, Zhihui; Yin, Yanlin; Chang, Qun; Sun, Guanqun; Lin, Jiahui; Dai, Yalei
• Is Part Of: Cell proliferation. Volume 50:Number 2(2017:Apr.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-11-23
• Subjects: Cell proliferation -- Periodicals; 571.84
• DOI: 10.1111/cpr.12319
• Languages: English
• ISSNs: 0960-7722

Abstract

Abstract: Objectives: To reveal whether B‐myb is involved in preventing senescence of vascular endothelial cells, and if so, to identify possible mechanisms for it. Materials and methods: C57/BL6 male mice and primary human aortic endothelial cells (HAECs) were used. Bleomycin was applied to induce stress‐related premature senescence. B‐myb knockdown was achieved using an siRNA technique and cell senescence was assessed using the senescence‐associated β‐galactosidase (SA‐β‐gal) assay. Intracellular reactive oxygen species (ROS) production was analysed using an ROS assay kit and cell proliferation was evaluated using KFluor488 EdU kit. Capillary tube network formation was determined by Matrigel assay. Expressions of mRNA and protein levels were detected by real‐time PCR and western blotting. Results: B‐myb expression significantly decreased, while p53 and p21 expressions increased in the aortas of aged mice. This expression pattern was also found in replicative senescent HAECs and senescent HAECs induced by bleomycin. B‐myb knockdown resulted in upregulation of p22 phox, ROS accumulation and cell senescence of HAECs. Downregulation of B‐myb significantly inhibited cell proliferation and capillary tube network formation and activated the p53/p21 signalling pathway. Blocking ROS production or inhibiting p53 activation remarkably attenuated SA‐β‐gal activity and delayed cell senescence induced by B‐myb‐silencing. Conclusion: Downregulation of B‐myb induced senescence by upregulation of p22 phox and activation of the ROS/p53/p21 pathway, in our vascular endothelial cells, suggesting that B‐myb may be a novel candidate for regulating cell senescence to protect against endothelial senescence‐related cardiovascular diseases.