Automated quantification of apoptosis in B‐cell chronic lymphoproliferative disorders: a prognostic variable obtained with the Cell‐Dyn Sapphire (Abbott) automated hematology analyzer. (2nd June 2014)

• Record Type: Journal Article
• Authors: Fumi, M.; Martins, D.; Pancione, Y.; Sale, S.; Rocco, V.
• Is Part Of: International journal of laboratory hematology. Volume 36:Number 6(2014:Dec.)
• Page Start: 628
• Page End: 635
• Publication Date: 2014-06-02
• Subjects: Lymphoproliferative -- Sapphire -- smudge cells -- Gumprecht -- Iodide -- propidium; Hematology -- Periodicals; Blood -- Diseases -- Periodicals; Hematology -- Periodicals; 616.15005
• DOI: 10.1111/ijlh.12198
• Languages: English
• ISSNs: 1751-5521

Abstract

Summary: Introduction: B‐chronic lymphocytic leukemia CLL, a neoplastic clonal disorder with monomorphous small B lymphocytes with scanty cytoplasm and clumped chromatin, can be morphologically differentiated in typical and atypical forms with different prognosis: Smudge cells (Gumprecht's shadows) are one of the well‐known features of the typical CLL and are much less inconsistent in other different types CLPD. Abbott Cell‐Dyn Sapphire uses the fluorescence after staining with the DNA fluorochrome propidium iodide for the measurement of nucleated red blood cells (NRBCs) and nonviable cells (FL3+ cell fraction): We have studied the possible correlation between presence and number of morphologically identifiable smudge cells on smears and the percentage of nonviable cells produced by Cell‐Dyn Sapphire. Methods: 305 blood samples from 224 patients with B‐cell lymphoproliferative disorders and 40 healthy blood donors were analyzed by CBC performed by Cell‐Dyn Sapphire, peripheral blood smear, and immunophenotype characterization. Result: FL3+ fraction in CLPD directly correlated with the percentage of smudge cells and is significantly increased in patients with typical B‐CLL. This phenomenon is much less evident in patients with atypical/mixed B‐CLL and B‐NHL. Conclusion: In small laboratories without FCM and cytogenetic, smudge cells%, can be utilized as a preliminary diagnostic and prognostic tool in differential diagnosis of CLPD.