Study of the genotoxic and cytotoxic effects of the α-, β-, and γ- Hexachlorocyclohexane isomers in human lymphocyte cells using the cytokinesis-block micronucleus assay. (2nd January 2017)

• Record Type: Journal Article
• Authors: Ennaceur, Soukaina
• Is Part Of: Drug and chemical toxicology. Volume 40:Number 1(2017:Jan.)
• Page Start: 85
• Page End: 89
• Publication Date: 2017-01-02
• Subjects: HCH isomers -- micronucleus test -- human lymphocytes -- genotoxicity -- cytotoxicity; Toxicology -- Periodicals; Drugs -- Toxicology -- Periodicals; Toxicology, Experimental -- Periodicals; 615.9005
• DOI: 10.1080/01480545.2016.1175008
• Languages: English
• ISSNs: 0148-0545

Abstract

Abstract: The genotoxic potential of hexachlorocyclohexane (HCH) isomers (α-, β-, and γ-) which are organochlorine pesticides was tested in peripheral blood lymphocyte cultures from two donors by using the cytokinesis-block micronucleus assay. Micronucleus (MN) frequency, binucleated cells with micronucleus (BNMN), and cytokinesis-blocked proliferation index (CBPI) were determined as genotoxic and cytotoxic endpoints. At the concentration ranges tested (12.5–100 μg.L −1 ), all HCH isomers induced dose-dependent cytotoxic effects, γ-HCH being the most toxic. This isomer was also able to induce significant increase in MN frequency and BNMN cells indicating a genotoxic potential at 50 and 100 μg.L −1 . The genotoxic test of β-HCH showed a positive induction of MN and BNMN cells at the highest concentration of 100 μg.L −1 and a significant cytotoxicity at 50 μg.L −1 . Under the experimental condition used, α-HCH was unable to induce any significant increase in MN frequency confirming that α-HCH is a non-genotoxic agent.