Identification of bioactive compounds with GC–Q-TOF–MS in the extracts from Clinacanthus nutans using subcritical carbon dioxide extraction. Issue 5 (24th March 2017)

• Record Type: Journal Article
• Authors: Alam, Md. Ariful; Zaidul, I. S. M.; Ghafoor, Kashif; Ferdosh, Sahena; Ali, Md. Eaqub; Mirhosseini, H.; Kamaruzzaman, B. Y.; Jahurul, M. H. A.; Khatib, A.
• Is Part Of: Separation science and technology. Volume 52:Issue 5(2017)
• Page Start: 852
• Page End: 863
• Publication Date: 2017-03-24
• Subjects: α-glucosidase inhibitory activity -- antioxidant -- Clinacanthus nutans -- subcritical carbon dioxide extraction; Separation (Technology) -- Periodicals; 660.284205
• DOI: 10.1080/01496395.2016.1271342
• Languages: English
• ISSNs: 0149-6395

Abstract

Abstract: Subcritical carbon dioxide Soxhlet extraction of biologically active compounds from Clincanthus nutans was investigated by full factorial design to identify and optimize the factors (particle size and co-solvent) affecting extract yield, antioxidant activity, total phenolic content, total flavonoid content, and α-glucosidase inhibitory activity. An average of 3.103% yield, 98.90% antioxidant activity, 49.40 mg/g (GAE) TPC, 43.76 mg/g (RE), and 88.58% AGI activity can be achieved using the optimum levels of independent variables. The GC-Q-TOF MS identification of optimized extract shown that different classes of phytoconstituents were successfully separated by CO2 -Soxhlet to produce potential antioxidant and α-glucosidase inhibitory activity.