The complete mitochondrial genome of Diaphorina citri (Hemiptera: Psyllidae) and phylogenetic analysis. (February 2017)

• Record Type: Journal Article
• Authors: Xiong, Xiao; Wu, Liuyu; Xin, Tianrong; Wang, Jin; Zou, Zhiwen; Xia, Bin
• Is Part Of: Biochemical systematics and ecology. Volume 70(2017)
• Page Start: 230
• Page End: 238
• Publication Date: 2017-02
• Subjects: Diaphorina citri kuwayama -- Mitochondrial genome -- Psylloidea -- Phylogenetic analysis; Chemotaxonomy -- Periodicals; Biochemical variation -- Periodicals; Ecology -- Periodicals; Biochemistry -- Periodicals; Ecology -- Periodicals; Chimiotaxinomie -- Périodiques; Variation biochimique -- Périodiques; Écologie -- Périodiques; 578.012
• DOI: 10.1016/j.bse.2016.12.008
• Languages: English
• ISSNs: 0305-1978

Abstract

Abstract: The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) is the most serious pest of citrus as the vector of Huanglongbing (HLB), the citrus greening disease. In this study, the complete mitochondrial genome (mitogenome) of D . citri has been sequenced and annotated, and a comparative analysis is provided with known Psylloidea species. The mitogenome of D. citri is a typical circular molecular of 15, 038 bp in length with an A + T content of 74.56%, contains the typical 37 genes and the gene order is identical to the other Psylloidea mitogenomes. The nucleotide composition and codon usage of D. citri are similar to the four Psylloidea species. All protein-coding genes (PCGs) use standard initiation codons (TAN), stop with TAA and TAG except ND2 and ND5 which stop with incomplete termination codon T. All tRNAs have the typical clover-leaf structure, with the exception of trnS1 lacking the dihydrouridine (DHU) arm. The control region is located between rrnL and the trnI gene with the highest A + T content among the five Psylloidea species. Phylogenetic analysis is conducted based on the 13 PCGs or/and 2 rRNAs of 23 Sternorrhycha mitogenomes. Both maximum likelihood (ML) and Bayesian inference (BI) analysis suggest a clear relationship of Psylloidea, Aleyrodoidea and Aphidoidea within Sternorrhycha, which support the traditional morphological classification. Highlights: The mitochondrial genome of Diaphorina citri is sequenced and annotated. A comparative analysis is provided with known Psylloidea species. Phylogenetic analysis based on mitochondrial genomes of Sternorrhycha support the traditional morphological classification. COI sequence diversity can be used as a DNA barcoding to identify closely related species for the lowest evolutionary rate.