Reliable collection of Caspian brown trout (Salmo trutta caspius) sperm using a catheter. (15th July 2016)

• Record Type: Journal Article
• Authors: Aramli, MS; Golshahi, K; Banan, A; Sotoudeh, E
• Is Part Of: Reproduction in domestic animals. Volume 51:Number 5(2016)
• Page Start: 831
• Page End: 834
• Publication Date: 2016-07-15
• Subjects: Animal breeding -- Periodicals; Veterinary pathology -- Periodicals; Livestock -- Breeding -- Periodicals; 636.082
• DOI: 10.1111/rda.12740
• Languages: English
• ISSNs: 0936-6768

Abstract

Contents: The traditional stripping procedure for collecting fish semen is associated with the risk of urine contamination, which may significantly affect semen quality and quantity. The use of a catheter as an alternative method for semen collection may overcome this problem. Therefore, this study compared Caspian brown trout ( Salmo trutta caspius ) semen parameters (i.e. sperm density, seminal plasma osmolality, motility parameters of spermatozoa analysed using computer‐assisted sperm analysis and fertility) between the traditional stripping method and the use of a catheter. All parameter values of the semen collected with a catheter were significantly higher ( p < .05; density = 7.67 ± 1.02 × 10 9 ml −1 and osmolality = 279.28 ± 32.84 mOsm kg −1 ) than those collected with stripping method (density = 4.85 ± 0.47 × 10 9 ml −1 and osmolality = 216.42 ± 20.75 mOsm kg −1 ). Semen collected with a catheter was characterized by higher spermatozoa motility compared with sperm collected via stripping. Similarly, the fertilization ability of sperm collected with a catheter was significantly greater ( p < .05) than sperm collected with the traditional stripping method. In conclusion, collection of sperm with a catheter was shown to effectively reduce urine contamination and is therefore recommended for the collection of Caspian brown trout sperm.