Simultaneous conversion of free fatty acids and triglycerides to biodiesel by immobilized Aspergillus oryzae expressing Fusarium heterosporum lipase. Issue 3 (15th December 2016)

• Record Type: Journal Article
• Authors: Amoah, Jerome; Quayson, Emmanuel; Hama, Shinji; Yoshida, Ayumi; Hasunuma, Tomohisa; Ogino, Chiaki; Kondo, Akihiko
• Is Part Of: Biotechnology journal. Volume 12:Issue 3(2017)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-12-15
• Subjects: Biodiesel -- Bioprocess -- Esterification -- Immobilized whole cell biocatalysis -- Transesterification; Biotechnology -- Periodicals; 660.605
• DOI: 10.1002/biot.201600400
• Languages: English
• ISSNs: 1860-6768

Abstract

Abstract: The presence of high levels of free fatty acids (FFA) in oil is a barrier to one‐step biodiesel production. Undesirable soaps are formed during conventional chemical methods, and enzyme deactivation occurs when enzymatic methods are used. This work investigates an efficient technique to simultaneously convert a mixture of free fatty acids and triglycerides (TAG). A partial soybean hydrolysate containing 73.04% free fatty acids and 24.81% triglycerides was used as a substrate for the enzymatic production of fatty acid methyl ester (FAME). Whole‐cell Candida antarctica lipase B‐expressing Aspergillus oryzae, and Novozym 435 produced only 75.2 and 73.5% FAME, respectively. Fusarium heterosporum lipase‐expressing A. oryzae produced more than 93% FAME in 72 h using three molar equivalents of methanol. FFA and TAG were converted simultaneously in the presence of increasing water content that resulted from esterification. Therefore, F. heterosporum lipase with a noted high level of tolerance of water could be useful in the industrial production of biodiesel from feedstock that has high proportion of free fatty acids. Abstract : The high free fatty acids (FFA) in oil has been a hurdle in one‐step biodiesel production. A lipase with both transesterification and esterification activity and has high water tolerance was successfully used for the conversion. This technique can be used for industrial production of biodiesel from waste cooking oils and microbial oils without pre‐treatment.