TNF‐α‐induced IL‐6 and MMP‐9 expression in immortalized ameloblastoma cell line established by hTERT. (21st November 2016)

• Record Type: Journal Article
• Authors: Ohta, K; Naruse, T; Ishida, Y; Shigeishi, H; Nakagawa, T; Fukui, A; Nishi, H; Sasaki, K; Ogawa, I; Takechi, M
• Is Part Of: Oral diseases. Volume 23:Number 2(2017)
• Page Start: 199
• Page End: 209
• Publication Date: 2016-11-21
• Subjects: ameloblastoma -- human telomerase reverse transcriptase -- TNF‐α -- IL‐6 -- MMP‐9; Mouth -- Diseases -- Research -- Periodicals; 617.522
• DOI: 10.1111/odi.12594
• Languages: English
• ISSNs: 1354-523X

Abstract

Abstract : Objective: Ameloblastoma (AM) shows locally invasive behaviour. However, biological investigations regarding regulation of gene expression associated with AM pathological features are difficult to perform, because AM cells can be passaged for a few generations due to senescence. We report a newly established immortalized AM cell line, AMB cells, by transfection with human telomerase reverse transcriptase (hTERT). Furthermore, we examined whether TNF‐ α modulates bone resorption‐related genes, IL‐6 and MMP‐9 in cooperation with TGF‐ β or IFN‐ γ . Materials and methods: Following transfection of an hTERT expression vector into AM cells using a non‐viral method, the effects of cytokines on the expressions of IL‐6 and MMP‐9 mRNA were examined using real‐time PCR. TNF‐ α ‐induced NF‐ κ B activity was examined by western blotting and transcription factor assays. Results: AMB cells continued to grow for more than 100 population doublings. Stimulation with TNF‐ α increased IL‐6 and MMP‐9 mRNA expressions, as well as NF‐ κ B activation. Furthermore, TGF‐ β and IFN‐ γ dramatically increased TNF‐ α ‐mediated expressions of MMP‐9 and IL‐6 mRNA, respectively, while those responses were suppressed by NF‐ κ B inhibitor. Conclusion: We established an immortalized AM cell line by hTERT transfection. TNF‐ α ‐mediated regulation of MMP‐9 and IL‐6 via NF‐ κ B may play an important role in the pathological behaviour of AMs, such as bone resorption.