Peptide hydrogel in vitro non‐inflammatory potential. (19th December 2016)

• Record Type: Journal Article
• Authors: Markey, A.; Workman, V. L.; Bruce, I. A.; Woolford, T. J.; Derby, B.; Miller, A. F.; Cartmell, S. H.; Saiani, A.
• Other Names: Alemán Carlos guestEditor.; Hamley Ian W. guestEditor.; Reches Meital guestEditor.
• Is Part Of: Journal of peptide science. Volume 23:Number 2(2017)
• Page Start: 148
• Page End: 154
• Publication Date: 2016-12-19
• Subjects: peptide -- hydrogel -- monocytes -- cell culture -- inflammatory response; Peptides -- Periodicals; Peptides -- Periodicals; 572.65
• DOI: 10.1002/psc.2940
• Languages: English
• ISSNs: 1075-2617

Abstract

Abstract : Peptide‐based hydrogels have attracted significant interest in recent years as these soft, highly hydrated materials can be engineered to mimic the cell niche with significant potential applications in the biomedical field. Their potential use in vivo in particular is dependent on their biocompatibility, including their potential to cause an inflammatory response. In this work, we investigated in vitro the inflammatory potential of a β ‐sheet forming peptide (FEFEFKFK; F: phenylalanine, E: glutamic acid; K: lysine) hydrogel by encapsulating murine monocytes within it (3D culture) and using the production of cytokines, IL‐ β, IL‐6 and TNF α, as markers of inflammatory response. No statistically significant release of cytokines in our test sample (media + gel + cells) was observed after 48 or 72 h of culture showing that our hydrogels do not incite a pro‐inflammatory response in vitro . These results show the potential biocompatibility of these hydrogels and therefore their potential for in vivo use. The work also highlighted the difference in monocyte behaviour, proliferation and morphology changes when cultured in 2D vs. 3D. © 2016 The Authors Journal of Peptide Science published by European Peptide Society and John Wiley & Sons Ltd. Abstract : In vitro 3D culture of murine monocytes in peptide hydrogel shows that hydrogel does not incite a pro‐inflammatory response.