Development of poly(lactide‐co‐glycolide) nanoparticles functionalized with a mitochondria penetrating peptide. (17th January 2017)

• Record Type: Journal Article
• Authors: Selmin, Francesca; Magri, Giulia; Gennari, Chiara G.M.; Marchianò, Silvia; Ferri, Nicola; Pellegrino, Sara
• Other Names: Alemán Carlos guestEditor.; Hamley Ian W. guestEditor.; Reches Meital guestEditor.
• Is Part Of: Journal of peptide science. Volume 23:Number 2(2017)
• Page Start: 182
• Page End: 188
• Publication Date: 2017-01-17
• Subjects: conformational study -- hybrid polymers -- mitochondria penetrating peptide -- nanoparticles -- poly(lactide‐co‐glycolide) -- polymer‐peptide; Peptides -- Periodicals; Peptides -- Periodicals; 572.65
• DOI: 10.1002/psc.2952
• Languages: English
• ISSNs: 1075-2617

Abstract

Abstract : The development of mitochondria‐targeting cell permeable vectors represents a promising therapeutic approach for several diseases, such as cancer and oxidative pathologies. Nevertheless, access to mitochondria can be difficult. A new hybrid material composed by poly(lactide‐co‐glycolide) (PLGA) functionalized with a 6‐mer mitochondria penetrating peptide (MPP), consisting in alternating arginine and unnatural cyclohexylalanine, was developed. Circular dichroism, FT‐IR and DSC studies indicated that the conjugation of the peptide with the polymer led to the obtainment of a more rigid material with respect to both PLGA and MPP as such. In particular, a conformational rearrangement to a helical structure was observed for MPP. MPP– PLGA conjugates were used for the preparation of nanoparticles that showed no cytotoxicity in MTT assay, suggesting their putative use for future studies on mitochondria targeting. Copyright © 2017 European Peptide Society and John Wiley & Sons, Ltd. Abstract : A new hybrid material composed by poly(lactide‐co‐glycolide) (PLGA) functionalized with a 6‐mer mithochondria penetrating peptide (MPP) was developed. A conformational transition to a helical structure was observed for MPP upon conjugation with the polymer. This new rigid material was used for the preparation of cell compatible nanoparticles.