TLR4 acts as a death receptor for ultraviolet radiation (UVR) through IRAK‐independent and FADD‐dependent pathway in macrophages. Issue 12 (29th November 2016)

• Record Type: Journal Article
• Authors: Zhou, Hua; Harberts, Erin; Fishelevich, Rita; Gaspari, Anthony A.
• Other Names: Wolf Peter guestEditor.; de Gruijl Frank guestEditor.
• Is Part Of: Experimental dermatology. Volume 25:Issue 12(2016)
• Page Start: 949
• Page End: 955
• Publication Date: 2016-11-29
• Subjects: death receptor -- IRAK -- macrophages -- MyD88 -- Toll‐like Receptor 4 (TLR4) -- ultraviolet radiation; Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.13222
• Languages: English
• ISSNs: 0906-6705

Abstract

Abstract: UVR‐induced apoptosis in cutaneous antigen presenting cells (APC) causes systemic immune suppression and is dependent on TLR4/MyD88 signalling, but the apoptotic signalling pathways have not been defined. Macrophages pretreated with lipopolysaccharide (LPS) were unresponsive to subsequent LPS treatment, however, but were susceptible to UVR‐induced apoptosis. Macrophage survival and apoptotic events after UVR were also unaffected by treatment with TLR4 antagonists, a blocking IgG or a TLR4 analog antagonist, suggesting that UVR cell death is independent of a soluble ligand. After UVR, IRAK4 KDKI (catalytically inactive IRAK4) and wild‐type (WT) macrophages show equivalent levels of survival, as measured by MTT assay, and apoptosis, as measured by cleaved caspase‐3. Furthermore, in macrophages from both mice, UVR activated caspase‐8 and PARP, while inactivating Rip3. This finding is supported by a lack of IRAK1 degradation after UVR, compared to treatment with TLR2 or TLR4 agonists. UVR induced association of MyD88 with FADD, an extrinsic apoptotic pathway protein, but not IRAK4. UVR‐induced migration of FADD to the cell membrane of WT macrophages, but not MyD88 −/− macrophages, was observed (confocal microscopy). Co‐immunoprecipitation using an epitope‐tagged MyD88 revealed that FADD, but not TRADD, was recruited to MyD88 within 30 minutes of UVR exposure. UVR engages TLR4/MyD88 as a death signalling complex, rather than the classical inflammatory signalling pathway triggered by PAMP recognition of TLR4. These studies provide the rationale for the future development of topical TLR4 modulating therapies to interfere with this UVB‐mediated apoptosis and the associated negative consequences of immune suppression.