Detection of fluticasone propionate in horse plasma and urine following inhaled administration. Issue 5 (18th April 2012)

• Record Type: Journal Article
• Authors: Gray, Bobby P.; Biddle, Simon; Pearce, Clive M.; Hillyer, Lynn
• Is Part Of: Drug testing and analysis. Volume 5:Issue 5(2013:May)
• Page Start: 306
• Page End: 314
• Publication Date: 2012-04-18
• Subjects: fluticasone propionate -- inhaled -- horse -- plasma -- urine; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.1329
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract : Fluticasone propionate (FP) is an anti‐inflammatory agent with topical and inhaled applications commonly used in the treatment of asthma in steroid‐dependent individuals. The drug is used in racehorses to treat Inflammatory Airway Disease; this work was performed in order to advise on its use and detect potential misuse close to racing. Methods were developed for the extraction and analysis of FP from horse plasma and a carboxylic acid metabolite (FP‐17βCOOH) from horse urine. The methods utilize ultra high performance liquid chromatography coupled to tandem mass spectrometry (UPLC‐MS/MS) in order to detect the extremely low concentrations of analyte present in both matrices. The developed methods were used to analyse plasma and urine samples collected following inhaled administration of FP to six thoroughbred horses. FP was detected in plasma for a minimum of 72 h post‐administration and FP‐17βCOOH was detected in urine for approximately 18 h post‐administration. The results show that it is possible to detect FP in the horse following inhaled administration. Copyright © 2012 John Wiley & Sons, Ltd. Abstract : Methods are presented for the sensitive detection of fluticasone propionate in horse plasma and a carboxylic acid metabolite in horse urine. Following an inhaled administration of FP, FP was detected in plasma for a minimum of 72 hours and FP‐17βCOOH was detected in urine for approximately 18 hours. We believe this to be the first reported detection of FP‐COOH in the horse.