Capturing native interactions: intrinsic methods to study chromatin conformation. Issue 12 (9th December 2016)

• Record Type: Journal Article
• Authors: Rowley, M Jordan; Corces, Victor G
• Is Part Of: Molecular systems biology. Volume 12:Issue 12(2016:Dec.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-12-09
• Subjects: Molecular biology -- Periodicals; Systems biology -- Periodicals; 572.8
• DOI: 10.15252/msb.20167438
• Languages: English
• ISSNs: 1744-4292

Abstract

Abstract : The 3D organization of chromatin controls gene expression through spatial interactions between genomic loci. FISH and 3C‐based methods that are commonly used to study chromatin organization utilize chemical crosslinking, a step that may introduce biases in detectable chromatin interactions. In their recent study, Papantonis and colleagues (Brant et al, 2016 ) developed alternative new methods of detecting chromatin contacts without the use of chemical crosslinking agents. These tools increase the resolution and confidence at which interactions can be identified, and may be informative for chromatin interaction dynamics. Abstract : Chemical crosslinking in 3C‐based methods may introduce biases in detectable chromatin interactions. In their recent study, Papantonis and colleagues (Brant et al, 2016 ) developed alternate new methods for detecting chromatin contacts without chemical crosslinking.