A cellular model for the investigation of depot specific human adipocyte biology. (2nd January 2017)

• Record Type: Journal Article
• Authors: Todorčević, Marijana; Hilton, Catriona; McNeil, Catriona; Christodoulides, Constantinos; Hodson, Leanne; Karpe, Fredrik; Pinnick, Katherine E.
• Is Part Of: Adipocyte. Volume 6:Number 1(2017)
• Page Start: 40
• Page End: 55
• Publication Date: 2017-01-02
• Subjects: abdominal -- adipogenesis -- body fat distribution -- gluteal -- human adipose tissue -- preadipocyte cell lines; Fat cells -- Periodicals; Adipocytes -- Periodicals; 611.018276
• DOI: 10.1080/21623945.2016.1277052
• Languages: English
• ISSNs: 2162-3945

Abstract

ABSTRACT: Upper-body adiposity is associated with increased metabolic disease risk, while lower-body adiposity is paradoxically protective. Efforts to understand the underlying mechanisms require appropriate and reproducible in vitro culture models. We have therefore generated immortalised (im ) human preadipocyte (PAD) cell lines derived from paired subcutaneous abdominal and gluteal adipose tissue. These cell lines, denotedim APAD andim GPAD display enhanced proliferation and robust adipogenic capacities. Differentiatedim APAD andim GPAD adipocytes synthesize triglycerides de novo and respond lipolytically to catecholamine-stimulation. Importantly the cells retain their depot-of-origin 'memory' as reflected by inherent differences in fatty acid metabolism and expression of depot-specific developmental genes. These features make these cell lines an invaluable tool for the in vitro investigation of depot-specific human adipocyte biology.