Development and validation of a new serum standard for the measurement of anti‐HEV antibodies in animals. Issue 3 (9th August 2016)

• Record Type: Journal Article
• Authors: Zhang, Yulin; Wang, Lin; Zeng, Hang; Gong, Wanyun; Wang, Ling; Zhuang, Hui
• Is Part Of: Journal of medical virology. Volume 89:Issue 3(2017)
• Page Start: 497
• Page End: 501
• Publication Date: 2016-08-09
• Subjects: HEV -- immune response -- diagnostic test -- animals -- ELISA quantitation; Virology -- Periodicals; 616
• DOI: 10.1002/jmv.24651
• Languages: English
• ISSNs: 0146-6615

Abstract

Abstract : Although hepatitis E virus (HEV) infection is a zoonosis, there is, currently, no standardized assay for quantitatively measuring anti‐HEV antibody levels in HEV animal reservoirs. In this study, anti‐HEV antibody positive serum from a rabbit (RS26) was calibrated by dose‐response parallel line assay using the World Health Organization (WHO) reference standard for anti‐HEV antibodies. After evaluating the stability of the RS26 serum, a quantification assay of anti‐HEV antibodies using RS26 as a standard was developed and evaluated for both reproducibility and suitability to field studies. The anti‐HEV antibody level of RS26 was calculated to be 39.54 IU/ml. A series of standard working serum for anti‐HEV antibodies consisted of five serum dilutions (3.950 IU/ml, 1.975 IU/ml, 0.986 IU/ml, 0.494 IU/ml, and 0.247 IU/ml). The quantitative assay using RS26 showed good range and reproducibility, effectively measuring the dynamics of anti‐HEV antibody concentrations in pigs and rabbits. In conclusion, we have developed a a stable and reproducible serum standard for the quantitation of anti‐HEV antibodies. J. Med. Virol. 89:497–501, 2017 . © 2016 Wiley Periodicals, Inc.