Dermal‐Epidermal Cross‐Talk: Differential Interactions With Microvascular Endothelial Cells. Issue 5 (10th November 2016)

• Record Type: Journal Article
• Authors: Bassino, Eleonora; Vallariello, Edoardo; Gasparri, Franco; Munaron, Luca
• Is Part Of: Journal of cellular physiology. Volume 232:Issue 5(2017:May)
• Page Start: 897
• Page End: 903
• Publication Date: 2016-11-10
• Subjects: Physiology -- Periodicals; Cell physiology -- Periodicals; 571.6
• DOI: 10.1002/jcp.25657
• Languages: English
• ISSNs: 0021-9541

Abstract

Abstract : The biological importance of circulatory blood supply and angiogenesis for hair growth is now well recognized, but the their regulatory mechanisms require more mechanistic investigation. In vitro cocultures and tricultures can be successfully employed to greatly improve our knowledge on paracrine crosstalk between cell types that populate the dermal‐epidermal interface and cutaneous vasculature. Here we report that human dermal fibroblasts (NHDF) promote viability and proliferation of microvascular endothelial cells (HMVEC), while HMVEC are not mitogenic for NHDF. In triculture setup, conditioned media (CM) obtained by cocultures (HMVEC/NHDF or HMVEC/follicle fibroblasts) differently modulate growth and proliferation of keratinocytes and alter the expression of metabolic and pro‐inflammatory markers. In conclusion, tricultures were successfully employed to characterize in vitro dermal‐epithelial and endothelial interactions and could integrate ex vivo and in vivo approaches by the use of high‐throughput and standardized protocols in controlled conditions. J. Cell. Physiol. 232: 897–903, 2017. © 2016 Wiley Periodicals, Inc. Abstract : In vitro model to study dermal epidermal cross‐talk‐tricultures were successfully employed to characterize in vitro endothelial, dermal, and epithelial interaction.