Immunomodulatory effects of Glycyrrhiza uralensis polysaccharide in glycinin-induced allergic mouse model. Issue 2 (4th March 2017)

• Record Type: Journal Article
• Authors: Chen, Jie; Gu, Xinli; Chen, Jianguo; Luo, Yan; Wang, Mengyuan; Yang, Hongyang; Guo, Xiao; Zhu, Xiaoqing
• Is Part Of: Food and agricultural immunology. Volume 28:Issue 2(2017)
• Page Start: 179
• Page End: 188
• Publication Date: 2017-03-04
• Subjects: Crude Glycyrrhiza uralensis polysaccharide -- glycinin -- immunotherapy -- allergy; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2016.1251393
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: This study aimed to investigate the therapeutic effects of Glycyrrhiza uralensis polysaccharide (GUP) on glycinin-induced allergic reactions in mouse model. Female BALB/c mice were pretreated for seven consecutive days with various concentrations of GUP aqueous solution or blank control. The mice were then orally administered with glycinin (10 mg/ml) three times and observed a week later. The survival rate and other indicators of the mice were measured, while IL-4, IL-13, plasma histamine and the lgE and IgG content in the serum were detected by enzyme-linked immunosorbent assay. The percentage of CD4+CD25+FoxP3+T cells was detected by flow cytometry. The results showed that IL-4, IL-13 and plasma histamine levels significantly decreased in the blood of mice treated with low GUP concentrations (20 mg/ml), while the specific glycinin-IgE, glycinin-IgG inhibition ratio and CD4+CD25+FoxP3+ T cells/CD4+ T cells ratio increased. However, the lowest, middle, high and the highest concentrations of GUP did not have significant effects on the immune reactions of glycinin-induced allergic mice. In conclusion, our study suggests that GUP is a promising agent to inhibit glycinin-induced allergy.