Myrica rubra leaves as a potential source of a dual 5-LOX/COX inhibitor. Issue 2 (4th March 2017)

• Record Type: Journal Article
• Authors: Langhansova, Lenka; Landa, Premysl; Kutil, Zsofia; Tauchen, Jan; Marsik, Petr; Rezek, Jan; Lou, Ji Dong; Yun, Zhu Li; Vanek, Tomas
• Is Part Of: Food and agricultural immunology. Volume 28:Issue 2(2017)
• Page Start: 343
• Page End: 353
• Publication Date: 2017-03-04
• Subjects: Myrica rubra -- anti-inflammatory -- cyclooxygenase 1 -- cyclooxygenase 2 -- 5-lipoxygenase; Immunochemistry -- Periodicals; Food -- Analysis -- Periodicals; Immunoassay -- Periodicals; 616.079
• DOI: 10.1080/09540105.2016.1272554
• Languages: English
• ISSNs: 0954-0105

Abstract

ABSTRACT: Myrica rubra Sieb. et Zucc. is a valuable fruit tree that is used in Chinese, Japanese and Taiwanese traditional medicine. We investigated the anti-inflammatory activity of M. rubra leaves extracted with four different solvents. Total phenolics were determined using the Folin–Ciocalteu method. Extracts were investigated for their inhibitory activity toward the pro-inflammatory enzymes cyclooxygenase-1 and -2 (COX-1, COX-2) and 5-lipoxygenase (5-LOX). The ethanol extract of M. rubra leaves demonstrated a strong inhibition of prostaglandin E2 (PGE2 ) biosynthesis catalyzed by both COX-1 (93.42%) and COX-2 (75.71%) and leukotriene B4 (LTB4 ) formation catalyzed by 5-LOX (82.72%). Further we identified selective COX-1 inhibition by the n -butanol and aqueous fractions of the ethanol extract (with an IC50 for COX-1 inhibition of 1.07 and 0.71 µg mL −1, respectively) and dual 5-LOX/COX inhibition by the ethyl acetate fraction (with an IC50 of 3.29 for COX-1, 2.54 for COX-2 and 8.30 µg mL −1 for 5-LOX).