Analysis of the factor XI variant Arg184Gly suggests a structural basis for factor IX binding to factor XIa. Issue 7 (15th July 2013)

• Record Type: Journal Article
• Authors: Geng, Y.; Verhamme, I. M.; Sun, M. F.; Bajaj, S. P.; Emsley, J.; Gailani, D.
• Is Part Of: Journal of thrombosis and haemostasis. Volume 11:Issue 7(2013)
• Page Start: 1374
• Page End: 1384
• Publication Date: 2013-07-15
• Subjects: blood coagulation -- factor IX -- factor XI -- factor XIa -- proteolysis; Thrombosis -- Periodicals; Hemostasis -- Periodicals; Blood coagulation disorders -- Periodicals; 616.1
• DOI: 10.1111/jth.12275
• Languages: English
• ISSNs: 1538-7933

Abstract

Summary: Background: A patient with factor XI (FXI) deficiency was reported with an Arg184Gly substitution in the FXI A3 domain. The A3 domain contains an exosite required for binding of FIX to activated FXI (FXIa). Objective: To test the effects of the Arg184Gly substitution on FIX activation, and to characterize the FIX‐binding site on FXIa. Methods: Recombinant FXIa and FIX variants were used to identify residues involved in FIX activation by FXIa. Analysis of the FXI structure was used to identify potential FIX‐binding sites. Results: The K m for FIX activation by FXIa‐Gly184 was approximately three‐fold higher than for FXIa, suggesting that Arg184 is part of the exosite. Arg184 and the adjacent residues, Ile183 and Asp185, contribute to charged and hydrophobic areas that are not present in the FXI homolog prekallikrein (PK). Replacing residues 183–185 with alanine abolished exosite activity, similarly to replacement of the entire A3 domain with the A3 domain from PK (FXIa/PKA3). Reintroducing FXI residues 183–185 into FXIa/PKA3 partially restored the exosite, and replacing residues 183–185 and 260–264 completely restored exosite function. FIX in which the Ω‐loop (residues 4–11) was replaced with the FVII Ω‐loop was activated poorly by FXIa, suggesting that the FIX Ω‐loop binds to FXIa. Conclusions: The results support a model in which the Ω‐loop of FIX binds to an area on FXIa composed of residues from the N‐terminus and C‐terminus of the A3 domain. These residues are buried in zymogen FXI, and must be exposed upon conversion to FXIa to permit FIX binding.