Urinary d‐lactate levels reflect renal function in aristolochic acid‐induced nephropathy in mice. (3rd April 2013)

• Record Type: Journal Article
• Authors: Huang, Tzu‐Chuan; Chen, Shih‐Ming; Li, Yi‐Chieh; Lee, Jen‐Ai
• Is Part Of: Biomedical chromatography. Volume 27:Number 9(2013:Sep.)
• Page Start: 1100
• Page End: 1106
• Publication Date: 2013-04-03
• Subjects: aristolochic acid nephropathy -- renal injury -- d‐lactate -- l‐lactate -- urine; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.2908
• Languages: English
• ISSNs: 0269-3879

Abstract

ABSTRACT: Urinaryd ‐lactate is highly correlated to diabetic nephropathy – a progressive kidney disease in renal glomeruli. In this study, we used a C3H/3e mouse model to investigate the relationship between urinaryd ‐lactate and aristolochic acid nephropathy where the glomerular structure is not affected. The nephropathy was induced using intravenous injections of aristolochic acid at a dosage of 10 mg/kg per day for 5 days and was characterized biochemically and histologically. The urinary excretions of proteins, N ‐acetyl‐ β ‐d ‐glucosaminidase and serum creatinine were determined and connected to histological conventional findings. Urinaryd ‐lactate was analyzed using column‐switching high‐performance liquid chromatography with fluorescence detection. The results showed a remarkable increase of urinary markers, including of urinary proteins and N ‐acetyl‐ β ‐d ‐glucosaminidase, and the histological examination confirmed a diagnosis of acute tubule necrosis. The ratio ofd ‐lactate to creatinine in the urine of aristolochic acid‐treated mice was approximately 36 times greater than that of the mice in the control group ( p < 0.05). The ratios for the two groups of mice were 311.00 ± 71.70 and 8.60 ± 1.80 µmol/mmol creatinine, respectively. These data confirm in vivo that urinaryd ‐lactate reflects renal injury conditions in aristolochic acid‐treated mice and may be a marker for the assessment of nephropathy. Copyright © 2013 John Wiley & Sons, Ltd.