Level of Granzyme B-positive T-regulatory cells is a strong predictor biomarker of acute Graft-versus-host disease after day +30 after allo-HSCT. (March 2017)

• Record Type: Journal Article
• Authors: Drokov, Mikhail Y.; Davydova, Julia O.; Kuzmina, Larisa A.; Galtseva, Irina V.; Kapranov, Nikolay M.; Vasilyeva, Vera A.; Dubnyak, Darya S.; Koroleva, Olga M.; Mikhalcova, Ekaterina D.; Popova, Natalia N.; Parovichnikova, Elena N.; Savchenko, Valery G.
• Is Part Of: Leukemia research. Volume 54(2017:Mar.)
• Page Start: 25
• Page End: 29
• Publication Date: 2017-03
• Subjects: Graft-versus-host disease -- Biomarker -- Predictor -- Granzyme B -- T-regulatory cells; Leukemia -- Periodicals; Leukemia -- Periodicals; Leucémie -- Périodiques; Leukemia; Periodicals; Electronic journals; Electronic journals; 616.9941905
• DOI: 10.1016/j.leukres.2017.01.014
• Languages: English
• ISSNs: 0145-2126

Abstract

Abstract: Acute Graft-versus-host-disease (aGVHD), the major complication and one of the main causes of poor outcomes of allogeneic hematopoietic stem cell transplantation (allo-HSCT). Nowadays there are no widely accepted cell, plasma or another biomarker that can be used for aGVHD prediction. We hypothesized that a level of Granzyme B-positive T regulatory (GZMB-positive Treg) cells on day + 30 after allo-HSCT could be the measure of immune response suppression and could predict aGVHD development after day +30. We applied a widespread and easy-to-perform method of multicolor flow cytometry to measure level of GZMB-positive Treg cells. Levels of GZMB-positive Tregs on day +30 after allo-HSCT were significantly higher in those patients who never developed aGVHD in comparison with the other group of patient with aGVHD after day +30 (p = 0.0229). We conclude that the level of GZMB-positive Treg cells is a strong predictor of acute Graft-versus-host disease after day +30 after allo-HSCT.