Control of early HIV-1 infection associates with plasmacytoid dendritic cell-reactive opsonophagocytic IgG antibodies to HIV-1 p24. (28th November 2016)

• Record Type: Journal Article
• Authors: Tjiam, M. Christian; Sariputra, Lucy; Armitage, Jesse D.; Taylor, James P.A.; Kelleher, Anthony D.; Tan, Dino B.A.; Lee, Silvia; Fernandez, Sonia; French, Martyn A.
• Is Part Of: AIDS. Volume 30:Number 18(2016)
• Publication Date: 2016-11-28
• Subjects: acute HIV infection -- HIV control -- HIV-1 p24 -- IgG subclasses -- opsonophagocytic antibodies; AIDS (Disease) -- Periodicals; Acquired Immunodeficiency Syndrome; AIDS (Disease); Periodicals; Periodicals; 616.9792005
• DOI: 10.1097/QAD.0000000000001242
• Languages: English
• ISSNs: 0269-9370

Abstract

Abstract : Objectives: We have previously demonstrated that HIV-1 p24-specific plasmacytoid dendritic cell-reactive opsonophagocytic antibody (PROAb) responses associate with control of chronic HIV infection. Here, we examined whether HIV-1 p24-specific PROAbs associate with control of early HIV infection and their relationship with HIV-1 p24-specific IgG subclasses. Methods: Plasma collected at 8 and 52 weeks following primary HIV-1 infection was obtained from antiretroviral therapy-naïve patients who were classified as 'good' (plasma HIV-1 RNA < 5000 copies/ml; n = 17) or 'poor' (HIV-1 RNA > 50 000 copies/ml; n = 15) controllers at week 52. HIV-1 p24-specific PROAb responses were assayed using a plasmacytoid dendritic cell line (Gen2.2), and HIV-1 p24-specific IgG3, IgG1 and IgG2 levels were assayed by ELISA. Results: HIV-1 p24-specific PROAb responses increased in 'good controllers' ( P = 0.01) but remained unchanged in 'poor controllers' between weeks 8 and 52. Of the HIV-1 p24-specific IgG subclasses measured, only IgG1 increased over this time period in 'good controllers' alone ( P = 0.003), which correlated with the increase in HIV-1 p24-specific PROAb responses ( r = 0.83, P < 0.0001). Depletion of IgG1 from IgG preparations of 'good controllers' resulted in the inhibition of HIV-1 p24-specific PROAb responses. In the total patient cohort, plasma HIV-1 RNA levels at week 52 correlated inversely with changes in HIV-1 p24-specific PROAb responses ( r = −0.37, P = 0.04) and IgG1 ( r = −0.51, P = 0.003) levels between weeks 8 and 52. Conclusion: Control of early HIV-1 infection was associated with an increase in HIV-1 p24-specific PROAb responses, which was mediated by HIV-1 p24-specific IgG1 antibodies. These findings provide further evidence that antibodies to HIV core proteins may contribute to control of HIV-1 infection. Abstract : Supplemental Digital Content is available in the text