Method-dependent Discrepancies in Fetal Hemoglobin Quantification in Patients With Hemoglobin S. Issue 5 (July 2016)

• Record Type: Journal Article
• Authors: Creary, Susan E.; Pyle-Eilola, Amy L.; Varga, Elizabeth; Cotten, Steven W.; S. Lorey, Thomas; Holmes, Daniel T.; Greene, Dina N.
• Is Part Of: Journal of pediatric hematology/oncology. Volume 38:Issue 5(2016)
• Publication Date: 2016-07
• Subjects: sickle cell -- capillary electrophoresis -- hemoglobin F; Pediatric hematology -- Periodicals; Tumors in children -- Periodicals; 618.9215
• DOI: 10.1097/MPH.0000000000000575
• Languages: English
• ISSNs: 1077-4114

Abstract

Abstract : Hemoglobin F (HbF) concentration is used in the diagnosis of certain hemoglobinopathies and accurate quantification is central to treatment of patients with sickle cell disease. The 2 most commonly used methods to quantify HbF are high performance liquid chromatography and capillary zone electrophoresis. This study reports discrepancies in HbF quantification between these methods when hemoglobin S is present in the sample. Clinicians and investigators should be mindful of the method used for HbF quantification when evaluating and treating patients who produce hemoglobin S.