Absence of Maternal Microchimerism in Regional Lymph Nodes of Children With Biliary Atresia. Issue 6 (June 2016)

• Record Type: Journal Article
• Authors: Engelmann, Carsten; Maelzer, Madeleine; Kreyenberg, Hermann
• Is Part Of: Journal of pediatric gastroenterology and nutrition. Volume 62:Issue 6(2016)
• Page Start: 804
• Page End: 807
• Publication Date: 2016-06
• Subjects: etiology -- Kasai operation -- portal lymphadenopathy -- single allele polymorphism; Children -- Nutrition -- Periodicals; Pediatric gastroenterology -- Periodicals; Infants -- Nutrition -- Periodicals; Nutrition disorders in children -- Periodicals; Child Nutrition -- Periodicals; Digestive System -- growth & development -- Periodicals; Gastrointestinal Diseases -- Periodicals; Infant Nutrition -- Periodicals; Nutrition Disorders -- Periodicals; Child; 618.923
• DOI: 10.1097/MPG.0000000000001093
• Languages: English
• ISSNs: 0277-2116

Abstract

ABSTRACT: Objective: Maternal lymphocytes have been cited as a potential cause of infantile biliary atresia (BA). When hepatoportoenterostomy is performed, locoregional lymphadenopathy is frequently encountered. Methods: We screened enlarged nodes from 6 consecutive nonsyndromatic BA patients (age: 68 days ± 18.9 days) for maternal elements using DNA fingerprinting with short tandem repeat analysis and quantitative real-time polymerase chain reaction for allelic (single nucleotide) sequence polymorphisms. Results: Although being partly positive in infants' peripheral blood, no maternal microchimerism could be demonstrated in any of the lymph nodes. Conclusion: This result challenges the hypothesis that maternal cells play a role in hilar lymphadenopathy of children with BA.