Therapeutic Whole-body Hypothermia Protects Remote Lung, Liver, and Kidney Injuries after Blast Limb Trauma in Rats. (June 2016)

• Record Type: Journal Article
• Authors: Ning, Jiaolin; Mo, Liwen; Yi, Bin; Gu, Jianteng; Lu, Kaizhi; Zhou, Yaying; Lai, Xinan; Zhao, Hailin; Ma, Daqing
• Is Part Of: Anesthesiology. Volume 124:Number 6(2016)
• Publication Date: 2016-06
• Subjects: Anesthesiology -- Periodicals; Anesthetics -- Periodicals; Anesthesia -- Periodicals; 617.9605
• DOI: 10.1097/ALN.0000000000001106
• Languages: English
• ISSNs: 0003-3022

Abstract

Abstract : Background: Severe blast limb trauma (BLT) induces distant multiple-organ injuries. In the current study, the authors determined whether whole-body hypothermia (WH) and its optimal duration (if any) afford protection to the local limb damage and distant lung, liver, and kidney injuries after BLT in rats. Methods: Rats with BLT, created by using chartaceous electricity detonators, were randomly treated with WH for 30 min, 60 min, 3 h, and 6 h (n = 12/group). Rectal temperature and arterial blood pressure were monitored throughout. Blood and lung, liver, and kidney tissue samples were harvested for measuring tumor necrosis factor-α, interleukin-6 and interleukin-10, myeloperoxidase activity, hydrogen sulfide, and biomarkers of oxidative stress at 6 h after BLT. The pathologic lung injury and the water content of the lungs, liver, and kidneys and blast limb tissue were assessed. Results: Unlike WH for 30 min, WH for 60 min reduced lung water content, lung myeloperoxidase activity, and kidney myeloperoxidase activity by 10, 39, and 28% (all P < 0.05), respectively. WH for 3 h attenuated distant vital organs and local traumatic limb damage and reduced myeloperoxidase activity, hydrogen peroxide and malondialdehyde concentration, and tumor necrosis factor-α and interleukin-6 levels by up to 49% (all P < 0.01). Likewise, WH for 6 h also provided protection to such injured organs but increased blood loss from traumatic limb. Conclusions: Results of this study indicated that WH may provide protection for distant organs and local traumatic limb after blast trauma, which warrants further study. Abstract : Whole-body hypothermia for 3 h immediately after injury in an experimental animal model provides multiorgan protection for traumatic injury after blast trauma.Supplemental Digital Content is available in the text.