Evaluating the autoinduction expression system and one‐step purification for high‐level expression and purification of gallbladder‐derived rhIL‐1Ra. (28th September 2016)

• Record Type: Journal Article
• Authors: Bashir, Hamid; Ahmed, Nadeem; Khan, Mohsin Ahmad; Zafar, Ahmad Usman; Tahir, Saad; Kanwal, Hina; Khan, Faidad; Rahman, Zia ur; Husnain, Tayyab
• Is Part Of: Biotechnology and applied biochemistry. Volume 64:Number 1(2017)
• Page Start: 20
• Page End: 26
• Publication Date: 2016-09-28
• Subjects: autoinduction expression system -- one‐step purification -- rhIL‐1Ra; Biotechnology -- Periodicals; Biochemical engineering -- Periodicals; Biochemistry -- Periodicals; Biochemistry -- Periodicals; Genetic Techniques -- Periodicals; Microbiological Techniques -- Periodicals; 660.6
• DOI: 10.1002/bab.1463
• Languages: English
• ISSNs: 0885-4513

Abstract

Abstract: Recent advancement in fermentation technologies resulted in the increased yields of recombinant proteins of biopharmaceutical and medicinal importance. Consequently, there is an important task to develop simple and easily scalable methods that can facilitate the production of high‐quality recombinant protein. Most of the recent reports described the expression of recombinant human IL‐1 receptor antagonist (rhIL‐1Ra) in Escherichia coli using isopropyl‐β‐d ‐thiogalacto pyranoside (IPTG), a nonmetabolizable and expensive compound, as an expression inducer. In this study, we describe the expression and one‐step purification of gallbladder‐derived rhIL‐1Ra by autoinduction in E. coli . This method includes special media that automatically induce the target protein expression from T7 promoter and allow the production of the target protein in high yield than the conventional IPTG induction method. In addition to fermentation process improvements, one‐step purification strategy is essential to make the process economical. We developed a single‐step cation exchange chromatography and obtained 300 mg/L of rhIL‐1Ra with 98% purity. Purified protein was characterized by SDS‐PAGE and Ion exchange HPLC (IEX‐HPLC). The described method can be used to scale up the production of rhIL‐1Ra and other recombinant proteins.