Characterization of small RNA populations in non-transgenic and aflatoxin-reducing-transformed peanut. (April 2017)

• Record Type: Journal Article
• Authors: Power, Imana L.; Dang, Phat M.; Sobolev, Victor S.; Orner, Valerie A.; Powell, Joseph L.; Lamb, Marshall C.; Arias, Renee S.
• Is Part Of: Plant science. Volume 257(2017)
• Page Start: 106
• Page End: 125
• Publication Date: 2017-04
• Subjects: RNAi RNA interference -- miRNA microRNA -- siRNA small interfering RNA -- sRNA small RNA; Peanut -- Groundnut -- Aflatoxin -- RNA interference -- Small RNAs -- miRNAs -- siRNAs; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2016.12.013
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: First report on sRNA sequencing of aflatoxin-reducing transformed peanut. Identified 2 siRNAs, 89 miRNAs, and 10 novel miRNAs associated with aflatoxin reduction. Identified accumulation differences of 21- and 24-nt sRNAs between seeds of control and transformed peanut. Abstract: Aflatoxin contamination is a major constraint in food production worldwide. In peanut ( Arachis hypogaea L.), these toxic and carcinogenic aflatoxins are mainly produced by Aspergillus flavus Link and A. parasiticus Speare. The use of RNA interference (RNAi) is a promising method to reduce or prevent the accumulation of aflatoxin in peanut seed. In this study, we performed high-throughput sequencing of small RNA populations in a control line and in two transformed peanut lines that expressed an inverted repeat targeting five genes involved in the aflatoxin-biosynthesis pathway and that showed up to 100% less aflatoxin B1 than the controls. The objective was to determine the putative involvement of the small RNA populations in aflatoxin reduction. In total, 41 known microRNA (miRNA) families and many novel miRNAs were identified. Among those, 89 known and 10 novel miRNAs were differentially expressed in the transformed lines. We furthermore found two small interfering RNAs derived from the inverted repeat, and 39 sRNAs that mapped without mismatches to the genome of A. flavus and were present only in the transformed lines. This information will increase our understanding of the effectiveness of RNAi and enable the possible improvement of the RNAi technology for the control of aflatoxins.