Evaluation of quantitative PCR for early diagnosis of Pseudomonas aeruginosa infection in cystic fibrosis: a prospective cohort study. (March 2017)

• Record Type: Journal Article
• Authors: Héry-Arnaud, G.; Nowak, E.; Caillon, J.; David, V.; Dirou, A.; Revert, K.; Munck, M.-R.; Frachon, I.; Haloun, A.; Horeau-Langlard, D.; Le Bihan, J.; Danner-Boucher, I.; Ramel, S.; Pelletier, M.-P.; Rosec, S.; Gouriou, S.; Poulhazan, E.; Payan, C.; Férec, C.; Rault, G.; Le Gal, G.; Le Berre, R.
• Is Part Of: Clinical microbiology and infection. Volume 23:Number 3(2017)
• Page Start: 203
• Page End: 207
• Publication Date: 2017-03
• Subjects: Cystic fibrosis -- Early diagnosis -- Pseudomonas aeruginosa -- Quantitative PCR -- Sensitivity -- Specificity; Medical microbiology -- Periodicals; Diagnostic microbiology -- Periodicals; Communicable diseases -- Periodicals; Infection -- Periodicals; 616.01
• DOI: 10.1016/j.cmi.2016.11.016
• Languages: English
• ISSNs: 1198-743X

Abstract

Abstract: Objectives: Early detection of Pseudomonas aeruginosa lung positivity is a key element in cystic fibrosis (CF) management. PCR has increased the accuracy of detection of many microorganisms. Clinical relevance of P. aeruginosa quantitative PCR (qPCR) in this context is unclear. Our aim was to determine P. aeruginosa qPCR sensitivity and specificity, and to assess the possible time saved by qPCR in comparison with standard practice (culture). Methods: A multicentre cohort study was conducted over a 3-year period in 96 patients with CF without chronic P. aeruginosa colonization. Sputum samples were collected at each visit. Conventional culture and two-step qPCR ( opr L qPCR and gyr B /ecf X qPCR) were performed for 707 samples. The positivity criteria were based on the qPCR results, defined in a previous study as follow: opr L qPCR positivity alone if bacterial density was <730 CFU/mL or opr L qPCR combined with gyr B /ecf X qPCR if bacterial density was ≥730 CFU/mL. Results: During follow up, 36 of the 96 patients with CF were diagnosed on culture as colonized with P. aeruginosa. This two-step qPCR displayed a sensitivity of 94.3% (95% CI 79.7%–98.6%), and a specificity of 86.3% (95% CI 83.4%–88.7%). It enabled P. aeruginosa acquisition to be diagnosed earlier in 20 patients, providing a median detection time gain of 8 months (interquartile range 3.7–17.6) for them. Conclusions: Implementing opr L and gyr B /ecf X qPCR in the management of patients with CF allowed earlier detection of first P. aeruginosa lung positivity than culture alone.