Conformational and Intermolecular Interaction Dynamics of Photolyase/Cryptochrome Proteins Monitored by the Time‐Resolved Diffusion Technique1. (9th January 2017)

• Record Type: Journal Article
• Authors: Kondoh, Masato; Terazima, Masahide
• Is Part Of: Photochemistry and photobiology. Volume 93:Number 1(2017)
• Page Start: 15
• Page End: 25
• Publication Date: 2017-01-09
• Subjects: Photochemistry -- Periodicals; Light -- Physiological effect -- Periodicals; 541.35
• DOI: 10.1111/php.12681
• Languages: English
• ISSNs: 0031-8655

Abstract

Abstract: Cryptochrome (CRY), a blue light sensor protein, possesses a similar domain structure to photolyase (PHR) that, upon absorption of light, repairs DNA damage. In this review, we compare the reaction dynamics of these systems by monitoring the reaction kinetics of conformational change and intermolecular interaction change based on time‐dependent diffusion coefficient measurements obtained by using the pulsed laser‐induced transient grating technique. Using this method, time‐dependent biomolecular interactions, such as transient dissociation reactions in solution, have been successfully detected in real time. Conformational change in (6‐4) PHR has not been detected after the photoexcitation by monitoring the diffusion coefficient. However, the repaired DNA dissociates from PHR with a time constant of 50 μs, which must relate to a minor conformational change. However, CRY exhibits a considerable diffusion change with a time constant of 400 ms, which indicates that the protein–solvent interaction is changed by the conformational change. The C‐terminal domain of CRY is shown to be responsible for this change. Abstract : Reaction dynamics of conformational changes and intermolecular interaction changes in cryptochrome and photolyase after photoexcitation are revealed by the pulsed laser‐induced transient grating technique.