LncRNA SPRY4-IT1 sponges miR-101-3p to promote proliferation and metastasis of bladder cancer cells through up-regulating EZH2. (1st March 2017)

• Record Type: Journal Article
• Authors: Liu, Dong; Li, Yawei; Luo, Gang; Xiao, Xingyuan; Tao, Dan; Wu, Xinchao; Wang, Miao; Huang, Chao; Wang, Liang; Zeng, Fuqing; Jiang, Guosong
• Is Part Of: Cancer letters. Volume 388(2017)
• Page Start: 281
• Page End: 291
• Publication Date: 2017-03-01
• Subjects: SPRY4-IT1 -- Bladder cancer -- miR-101-3p -- EZH2 -- Long noncoding RNA; Cancer -- Periodicals; Neoplasms -- Periodicals; Cancer -- Périodiques; Electronic journals; 616.994
• DOI: 10.1016/j.canlet.2016.12.005
• Languages: English
• ISSNs: 0304-3835

Abstract

Abstract: Emerging evidences have indicated that long non-coding RNAs (LncRNAs) play vital roles in cancer development and progression. Previous studies have suggested that overexpression of SPRY4-IT1 predicates poor prognosis and promotes tumor progress in several cancers. However, the underlying mechanism of SPRY4-IT1 in bladder cancer remains unknown. In this study, we found that SPRY4-IT1 knockdown induced inhibition of cell proliferation, cell migration and invasion ability, and caused promotion of apoptosis in bladder cancer both in vitro and in vivo. Mechanistically, knockdown of SPRY4-IT1 increased the expression of miR-101-3p and subsequently inhibited the expression of EZH2 at posttranscriptional level. Importantly, SPRY4-IT1 could directly interact with miR-101-3p and down-regulation of miR-101-3p efficiently reversed the suppression of EZH2 induced by SPRY4-IT1 shRNA. Thus, SPRY4-IT1 positively regulated the expression of EZH2 through sponging miR-101-3p, and played an oncogenic role in bladder cancer progression. Together, our study elucidates the role of LncRNA SPRY4-IT1 as a miRNA sponge in bladder cancer, and sheds new light on LncRNA-directed diagnostics and therapeutics in bladder cancer. Highlights: SPRY4-IT1 promotes tumor proliferation and metastasis in bladder cancer cells. SPRY4-IT1 functions as an miRNA sponge for miR-101-3p to de-repress the EZH2 expression. The SPRY4-IT1/miR-101-3p/EZH2 axis contributes to bladder cancer proliferation and metastasis. GA treatment induces repression of SPRY4-IT1 and could function as a SPRY4-IT1 inhibitor.