Development of a novel integrated process for co-production of β-galactosidase and ethanol using lactose as substrate. (April 2017)

• Record Type: Journal Article
• Authors: You, Shengping; Zhang, Jianye; Yin, Qingdian; Qi, Wei; Su, Rongxin; He, Zhimin
• Is Part Of: Bioresource technology. Volume 230(2017)
• Page Start: 15
• Page End: 23
• Publication Date: 2017-04
• Subjects: Integrated process -- Lactose -- β-Galactosidase -- Ethanol -- Kluyveromyces lactis; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2017.01.019
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: A novel integrated process for co-production of β-galactosidase and ethanol was built. A new mathematic model for a new recycling permeabilization was established. β-Galactosidase product was at 2.61 U/mg and ethanol product was at 33.8% (v/v). The novel integrated process might be a feasible strategy to scale up. Abstract: A novel integrated process was developed successfully for co-production of β-galactosidase and ethanol using lactose as substrate, containing fermentation ( Kluyveromyces lactis ), isolation, permeabilization (a new recycling process) and spray drying. Firstly, a new fed-batch strategy optimized co-produced β-galactosidase at 105.91 U/mL and ethanol at 32.16 mg/mL, 4.40-fold and 10.82-fold increase over the results from initial conditions, respectively. Then a new mathematic model for the recycling permeabilization was established successfully. As expected, the total cells sediment from isolation of the fed-batch culture was permeabilized completely by distilled ethanol from broth supernatant. More amazedly, the specific activity of β-galactosidase product by spray drying the permeabilized cells reached 2.61 U/mg, meeting the demand of commercial products. Furthermore, the ethanol product at 33.8% (v/v) was obtained from the novel integrated process, which could be applied for various applications. To conclude, the novel integrated process might be a feasible strategy to scale up for industrialization.