A beginner's guide to tissue clearing. (March 2017)

• Record Type: Journal Article
• Authors: Ariel, Pablo
• Is Part Of: International journal of biochemistry & cell biology. Volume 84(2017)
• Page Start: 35
• Page End: 39
• Publication Date: 2017-03
• Subjects: Tissue clearing -- Optical clearing -- Whole organ imaging -- Whole organism imaging -- Organoids; Biochemistry -- Periodicals; Cytology -- Periodicals; Biochemistry -- Periodicals; Cell Biology -- Periodicals; Biochimie -- Périodiques; Cytologie -- Périodiques; Biochimie; Cytologie; Biochemistry; Cytology; Ressource Internet (Descripteur de forme); Périodique électronique (Descripteur de forme); Periodicals; 572.05
• DOI: 10.1016/j.biocel.2016.12.009
• Languages: English
• ISSNs: 1357-2725

Abstract

Highlights: Tissue clearing allows imaging many millimeters deep in fixed tissue. Any research project using fixed samples more than a few tens of microns thick can benefit from tissue clearing. Sample size, the need for immunostaining and the need for lipid staining are key to selecting a clearing method. Abstract: The last decade has seen a proliferation of tissue clearing methods that render large biological samples transparent and allow unprecedented three-dimensional views of enormous volumes of tissue. For a scientist wondering whether these methods will be useful to address their research problems, it can be bewildering to sort through the ever-increasing number of papers introducing new clearing methods. Here, I provide a concise summary for the novice describing what tissue clearing is, which research problems it can be applied to, how to decide on a clearing method, and where the field is headed in the future.