SUMOylation of KLF4 promotes IL-4 induced macrophage M2 polarization. Issue 4 (16th February 2017)

• Record Type: Journal Article
• Authors: Wang, Kezhou; Zhou, Wei; Cai, Qi; Cheng, Jinke; Cai, Rong; Xing, Rong
• Is Part Of: Cell cycle. Volume 16:Issue 4(2017)
• Page Start: 374
• Page End: 381
• Publication Date: 2017-02-16
• Subjects: alternative polarization -- IL-4 -- Kruppel-like factor 4 (KLF4) -- macrophage -- SUMOylation; Cell cycle -- Periodicals; 571.84377
• DOI: 10.1080/15384101.2016.1269045
• Languages: English
• ISSNs: 1538-4101

Abstract

ABSTRACT: Macrophages, in response to different environmental cues, undergo the classical polarization (M1 macrophages) as well as the alternative polarization (M2 macrophages) that involve the functions of stimulus-specific transcription factors. Kruppel-like factor 4 (KLF4), a member of a subfamily of the zinc-finger class of DNA-binding transcription factors, plays as a critical regulator of macrophage polarization. KLF4 has been reported as a SUMOylated protein. In this study, we showed that SUMOylation of KLF4, is induced by IL-4 treatment in macrophages. IL4-induced KLF4 SUMOylation promotes RAW264.7 cells and bone marrow derived macrophages (BMDMs) to polarize into M2 subset. Thus, we identified an important post-translational modification (PTM), SUMOylation, plays a crucial role in regulating KLF4 activity during IL-4 induced macrophage M2 polarization. SUMOylation of KLF4 can be a potential therapeutic target in the resolution of inflammation.