Determination of dihydroartemisinic acid in Artemisia annua L. by gas chromatography with flame ionization detection. (18th September 2016)

• Record Type: Journal Article
• Authors: Tian, Na; Tang, Yuwei; Tian, Dongming; Liu, Zhonghua; Liu, Shuoqian
• Is Part Of: Biomedical chromatography. Volume 31:Number 3(2017:Mar.)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2016-09-18
• Subjects: Artemisia annua L -- dihydroartemisinic acid -- flame ionization detection -- gas chromatograhpy -- quantification; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.3824
• Languages: English
• ISSNs: 0269-3879

Abstract

Abstract: Dihydroartemisinic acid (DHAA) is the direct precursor to artemisinin, an effective anti‐malaria compound from Artemisia annua L. ( A. annua ), and it can be transformed to artemisinin without the catalysis of enzyme. A rapid and sensitive analysis of DHAA in A. annua is needed to screen excellent plant resources aimed to improve artemisinin production. In order to develop a rapid and sensitive determination method for DHAA in plant, the extraction and analysis conditions were extensively investigated in the present work. As a result, extraction of powdered A. annua leaves at 55°C for 50 min with chloroform resulted in the highest yield of DHAA, with a recovery of >98%. The precision of this gas chromatographic procedure ranged from 1.22 to 2.94% for intra‐day and from 1.69 to 4.31% for inter‐day, respectively. The accuracy was 99.55–103.02% for intra‐day and 98.86–99.98% for inter‐day, respectively. The measured LOQ and LOD values of the proposed method reached 5.00 and 2.00 μg/mL, respectively. Validation indicated the method was robust, quick, sensitive and adequate for DHAA analysis.