Bioactivity‐integrated ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry for the identification of nuclear factor‐κB inhibitors and β2 adrenergic receptor agonists in Chinese medicinal preparation Chuanbeipipa dropping pills. (11th March 2013)

• Record Type: Journal Article
• Authors: Dong, Linyi; Luo, Yi; Cheng, Binfeng; Zhang, Yaoshu; Zhang, Ning; Hou, Yuanyuan; Jiang, Min; Luo, Guoan; Bai, Gang
• Is Part Of: Biomedical chromatography. Volume 27:Number 8(2013:Aug.)
• Page Start: 960
• Page End: 967
• Publication Date: 2013-03-11
• Subjects: multiple‐bioactivity‐integrated fingerprinting -- β2 adrenergic receptor agonists -- nuclear factor‐κB inhibitors -- UPLC‐Q‐TOF MS/MS -- Chuanbeipipa dropping pills; Chromatographic analysis -- Periodicals; Biology -- Periodicals; Medicine -- Periodicals; Biology -- Periodicals; Chromatography -- methods -- Periodicals; Medicine -- Periodicals; 543.089
• DOI: 10.1002/bmc.2886
• Languages: English
• ISSNs: 0269-3879

Abstract

ABSTRACT: A simple and dual‐target method based on ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry combined with dual‐bioactive [nuclear factor‐ κ B (NF‐ κ B) and β 2 ‐adrenergic receptor] luciferase reporter assay systems was developed to rapidly characterize the chemical structure of various bioactive compounds of TCM preparations. Chuanbeipipa dropping pills, a traditional Chinese medicine preparation used for the clinical therapy of chronic obstructive lung disease and cough caused by bronchial catarrh, was analyzed with this method. Potential anti‐inflammatory and spasmolytic constituents were screened using NF‐ κ B and β 2 ‐adrenergic receptor activity luciferase reporter assay systems and simultaneously identified according to the time‐of‐flight mass spectrometry data. One β 2 ‐adrenergic receptor agonist (ephedrine) and two structural types of NF‐ κ B inhibitors (platycosides derivatives and ursolic acid derivatives) were characterized. Platycodin D3 and E were considered new NF‐ κ B inhibitors. Further cytokine and chemokine detection confirmed the anti‐inflammatory effects of the potential NF‐ κ B inhibitors. Compared with conventional fingerprints, activity‐integrated fingerprints that contain both chemical and bioactive details offer a more comprehensive understanding of the chemical makeup of plant materials. This strategy clearly demonstrated that multiple bioactivity‐integrated fingerprinting is a powerful tool for the improved screening and identification of potential multi‐target lead compounds in complex herbal medicines. Copyright © 2013 John Wiley & Sons, Ltd.