Comparative transcriptome analysis between resistant and susceptible tomato allows the identification of lncRNA16397 conferring resistance to Phytophthora infestans by co‐expressing glutaredoxin. (3rd February 2017)

• Record Type: Journal Article
• Authors: Cui, Jun; Luan, Yushi; Jiang, Ning; Bao, Hang; Meng, Jun
• Is Part Of: Plant journal. Volume 89:Number 3(2017)
• Page Start: 577
• Page End: 589
• Publication Date: 2017-02-03
• Subjects: comparative transcriptome -- tomato -- Phytophthora infestans -- lncRNA -- co‐expressing; Plant molecular biology -- Periodicals; Plant cells and tissues -- Periodicals; Botany -- Periodicals; 580
• DOI: 10.1111/tpj.13408
• Languages: English
• ISSNs: 0960-7412

Abstract

Summary: The rapid development of omics sequencing technology has facilitated the identification of thousands of long non‐coding (lnc)RNAs in plant species, but the role of lncRNAs in plant–pathogen interactions remains largely unexplored. We used comparative transcriptome analysis of Phytophthora infestans ‐resistant and ‐susceptible tomatoes to identify differentially expressed genes (DEGs) and lncRNAs (DELs), and examine lncRNA‐mRNA networks. A total of 1037 DEGs and 688 DELs were identified between P. infestans‐ resistant and ‐susceptible tomatoes. The co‐localization networks, including 128 DEGs and 127 DELs, were performed. We found that lncRNA16397 acted as an antisense transcript of SlGRX22 to regulate its expression, and also induced SlGRX21 expression when lncRNA16397 was overexpressed. In addition, disease symptoms and reactive oxygen species (ROS) accumulation in tomatoes overexpressing lncRNA16397 and SpGRX were fewer and lower than those in wild‐type after P. infestans infection. This result suggests that tomato lncRNA16397 induces SlGRX expression to reduce ROS accumulation and alleviate cell membrane injury, resulting in enhanced resistance to P. infestans . Our results provide insight into lncRNAs involved in the response of tomato to P. infestans infection, demonstrate that the lncRNA16397‐GRXs network is an important component of the P. infestans network in tomato, and provide candidates for breeding to enhance biotic stress‐resistance in tomato. Significance Statement: There are thousands of lncRNAs in plants, but their role in plant‐pathogen interactions remains largely unexplored. Here, by using comparative transcriptome analysis of Phytophthora infestans ‐resistant and susceptible tomato, we show that lncRNA16397 can induce gene expression to reduce ROS accumulation and alleviate cell membrane injury, thus enhancing resistance to P. infestans . We also pinpoint other candidate genes that can be used to breed for enhanced biotic stress‐resistance.