Testing for the erythropoiesis‐stimulating agent Sotatercept/ACE‐011 (ActRIIA‐Fc) in serum by means of Western blotting and LC‐HRMS. Issue 11 (13th October 2016)

• Record Type: Journal Article
• Authors: Walpurgis, Katja; Thomas, Andreas; Vogel, Matthias; Reichel, Christian; Geyer, Hans; Schänzer, Wilhelm; Thevis, Mario
• Is Part Of: Drug testing and analysis. Volume 8:Issue 11/12(2016:Nov./Dec.)
• Page Start: 1152
• Page End: 1161
• Publication Date: 2016-10-13
• Subjects: ActRIIA‐Fc -- affinity purification -- doping -- LC‐HRMS -- Western blotting; Drugs -- Analysis -- Periodicals; Drug testing -- Periodicals; Chemistry, Forensic -- Periodicals; 615.1901
• DOI: 10.1002/dta.2093
• Languages: English
• ISSNs: 1942-7603

Abstract

Abstract : Sotatercept (formerly ACE‐011) is a glycosylated, dimeric fusion protein composed of the extracellular domain of the human activin receptor type IIA (ActRIIA) and the Fc region of human IgG1. The protein‐based drug candidate acts as a ligand trap which competitively binds to activin A and other members of the transforming growth factor beta superfamily, thus blocking signalling through ActRIIA. Since the inhibition of activin A was found to significantly increase bone formation and quality, Sotatercept was originally developed for the treatment of diseases involving bone loss. But as the protein therapeutic also stimulates erythropoiesis by a mechanism independent of the EPO receptor, it has been evaluated for the treatment of anaemia in rare blood diseases such as beta thalassemia. Due to its positive effects on erythropoiesis and bone formation, Sotatercept may also be misused as performance‐enhancing agent in sports. Within this study, two complementary detection assays for Sotatercept and related ActRIIA‐Fc fusion proteins in serum samples were developed. While the first assay combines affinity purification and Western blotting to generically detect ActRIIA‐Fc fusion proteins irrespective of their amino acid sequence, the liquid chromatography‐high resolution mass spectrometry (LC‐HRMS) method is highly specific for proteolytic peptides originating from the receptor and Fc domain of Sotatercept. Both approaches can readily be modified to include other pharmaceutical proteins such as therapeutic antibodies, and serve as proof‐of‐concept for the capability of the approach to detect TGF‐β inhibitors and Fc fusion proteins in doping control serum samples. Copyright © 2016 John Wiley & Sons, Ltd. Abstract : Sotatercept is a novel erythropoiesis‐stimulating agent composed of the extracellular domain of the human activin receptor type IIA (ActRIIA) and the Fc fragment of human IgG1. Within this study, both a non‐targeted and targeted detection assay for Sotatercept and related ActRIIA‐fc fusion proteins were developed by using either Western blotting or tryptic digestion and LC‐HRMS in combination with upstream affinity purification. The LC‐HRMS method was successfully validated and found to be fit‐for‐purpose for an application in routine doping control analysis.