Comparison of immune responses and antioxidant status of different generations of growth‐selected Portunus trituberculatus families. (29th January 2016)

• Record Type: Journal Article
• Authors: Ren, Xianyun; Gao, Baoquan; Liu, Xianxi; Li, Jian; Liu, Ping
• Is Part Of: Aquaculture research. Volume 48:Number 3(2017)
• Page Start: 1315
• Page End: 1326
• Publication Date: 2016-01-29
• Subjects: inbreeding -- Portunus trituberculatus -- immune response -- antioxidant status; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12973
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: The purpose of this study was to investigate the effects of inbreeding on the immune responses and antioxidant status of Portunus trituberculatus juveniles. Results showed that inbreeding affected the total haemocyte count, and phagocytic, pro‐phenoloxidase (propo), phenoloxidase and antibacterial activities decreased after the seventh generation. Antioxidant status showed a similar pattern: total antioxidant capacity, superoxide dismutase (SOD) activity and GSH/GSSG in the cell‐free haemolymph and hepatopancreas decreased, while catalase activity in the cell‐free haemolymph increased ( P < 0.05). There were no significant differences in α2 ‐macroglobulin and bacteriolytic activities in the cell‐free haemolymph and glutathione peroxidase activity in the cell‐free haemolymph and hepatopancreas among nine inbreeding generations. Gene expression levels of proPO and crustin in haemocytes and SOD in haemocytes and hepatopancreas also decreased significantly as the inbreeding generations increased. The results suggest that a high level of inbreeding could severely reduce the physiological health of P. trituberculatus . Our obtained data would be particularly useful for P. trituberculatus breeding programmes.