Teneurin-2 (TENM2) deficiency induces UCP1 expression in differentiating human fat cells. (5th March 2017)

• Record Type: Journal Article
• Authors: Tews, D.; Fromme, T.; Keuper, M.; Hofmann, S.M.; Debatin, K.M.; Klingenspor, M.; Wabitsch, M.; Fischer-Posovszky, P.
• Is Part Of: Molecular and cellular endocrinology. Volume 443(2017)
• Page Start: 106
• Page End: 113
• Publication Date: 2017-03-05
• Subjects: Brown adipose tissue -- TENM2 -- Adipogenesis -- Obesity; adip adipocyte -- ACAA1 acetyl-CoA acyltransferase 1 -- ACACA acetyl-CoA carboxylase alpha -- ACOX1 acyl-CoA oxidase 1 -- CIDEA Cell Death-Inducing DFFA-Like Effector A -- CIDEC cell death inducing DFFA like effector c -- CPT1B Carnitine Palmitoyltransferase 1B -- dn deep neck -- ELOVL3 ELOVL fatty acid elongase 3 -- FCCP Carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone -- LHX8 LIM Homeobox 8 -- MYF5 Myogenic Factor 5 -- OCR oxygen consumption rate -- PGC1a Peroxisome proliferator-activated receptor gamma coactivator 1-alpha -- PPARg Peroxisome Proliferator Activated Receptor Gamma -- PRDM16 PR domain containing 16 -- pre preadipocytes -- sc subcutaneous -- SGBS Simpson-Golabi-Behmel syndrome -- TMEM26 transmembrane protein 26 -- TENM2 teneurin-2 -- TCAP teneurin c-terminal associated peptide -- ZIC1 Zic Family Member 1; Endocrinology -- Periodicals; Molecular biology -- Periodicals; Cytology -- Periodicals; Endocrinology -- Periodicals; Hormones -- Periodicals; Endocrinologie -- Périodiques; Cytology; Endocrinology; Molecular biology; Periodicals; 573.4
• DOI: 10.1016/j.mce.2017.01.015
• Languages: English
• ISSNs: 0303-7207

Abstract

Abstract: Under certain conditions UCP1 expressing adipocytes arise in white adipose tissue depots of both mice and humans. It is still not fully understood whether these cells differentiate de novo from specific progenitor cells or if they transdifferentiate from mature white adipocytes. Performing expression pattern analysis comparing adipocyte progenitor cells from deep and subcutaneous neck adipose tissue, we recently identified teneurin-2 (TENM2) enriched in white adipocyte progenitor cells. Here we tested whether TENM2 deficiency in adipocyte progenitor cells would lead to a brown adipocyte phenotype. By targeting TENM2 in SGBS preadipocytes using siRNA, we demonstrate that TENM2 knockdown induces both UCP1 mRNA and protein expression upon adipogenic differentiation without affecting mitochondrial mass. Furthermore, TENM2 knockdown in human SGBS adipocytes resulted in increased basal and leak mitochondrial respiration. In line with our previous observation these data suggest that TENM2 deficiency in human adipocyte precursors leads to induction of brown adipocyte marker genes upon adipogenic differentiation. Highlights: TENM2 is higher expressed in human white compared to brown preadipocytes. TENM2 deficiency in human preadipocytes leads to upregulation of UCP1 expression. This in turn results in increased basal and leak respiration rates.