Blue light irradiation suppresses dendritic cells activation in vitro. Issue 8 (23rd July 2013)

• Record Type: Journal Article
• Authors: Fischer, Michael R.; Abel, Manuela; Lopez Kostka, Susanna; Rudolph, Berenice; Becker, Detlef; von Stebut, Esther
• Is Part Of: Experimental dermatology. Volume 22:Issue 8(2013:Aug.)
• Page Start: 558
• Page End: 560
• Publication Date: 2013-07-23
• Subjects: atopic dermatitis -- blue light -- dendritic cells -- immunosuppression; Dermatology -- Periodicals; 616.5
• DOI: 10.1111/exd.12193
• Languages: English
• ISSNs: 0906-6705

Abstract

Abstract: Blue light is a UV‐free irradiation suitable for treating chronic skin inflammation, for example, atopic dermatitis, psoriasis, and hand‐ and foot eczema. However, a better understanding of the mode of action is still missing. For this reason, we investigated whether dendritic cells (DC) are directly affected by blue light irradiation in vitro . Here, we report that irradiation neither induced apoptosis nor maturation of monocyte‐derived and myeloid DC. However, subsequent DC maturation upon LPS/IFNγ stimulation was impaired in a dose‐dependent manner as assessed by maturation markers and cytokine release. Moreover, the potential of this DC to induce cytokine secretion from allogeneic CD4 T cells was reduced. In conclusion, unlike UV irradiation, blue light irradiation at high and low doses only resulted in impaired DC maturation upon activation and a reduced subsequent stimulatory capacity in allogeneic MLRs with strongest effects at higher doses.