Light enhanced the accumulation of total fatty acids (TFA) and docosahexaenoic acid (DHA) in a newly isolated heterotrophic microalga Crypthecodinium sp. SUN. (March 2017)

• Record Type: Journal Article
• Authors: Sun, Dongzhe; Zhang, Zhao; Mao, Xuemei; Wu, Tao; Jiang, Yue; Liu, Jin; Chen, Feng
• Is Part Of: Bioresource technology. Volume 228(2017)
• Page Start: 227
• Page End: 234
• Publication Date: 2017-03
• Subjects: Crypthecodinium sp. -- Total fatty acids -- DHA -- Starch -- Neutral lipids; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2016.12.077
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: A Crypthecodinium strain was isolated and named as Crypthecodinium sp. SUN. Light was firstly employed on Crypthecodinium and increased its TFA and DHA contents. The increase of TFA content was contributed by the decrease of starch. Light increased the specific growth rate of Crypthecodinium sp. SUN. Abstract: In the present study, light illumination was found to be efficient in elevating the total fatty acid content in a newly isolated heterotrophic microalga, Crypthecodinium sp. SUN. Under light illumination, the highest total fatty acid and DHA contents were achieved at 96 h as 24.9% of dry weight and 82.8 mg g −1 dry weight, respectively, which were equivalent to 1.46-fold and 1.68-fold of those under the dark conditions. The elevation of total fatty acid content was mainly contributed by an increase of neutral lipids at the expense of starches. Moreover, light was found to alter the cell metabolism and led to a higher specific growth rate, higher glucose consumption rate and lower non-motile cell percentage. This is the first report that light can promote the total fatty acids accumulation in Crypthecodinium without growth inhibition.